In order to access a running instance via a web browser or programmatically via the NCBI-BLAST Common URL API interface, one needs to provide credentials.

The user name is set to blast and the password is the AWS instance ID.

A web browser will prompt for credentials (see screenshot below); programmatic access will require authentication to be configured. For example, assuming the instance’s ID is i-01ef8ea68031299de and its public DNS is ec2-54-90-191-42.compute-1.amazonaws.com:

$ curl http://blast:i-01ef8ea68031299de@ec2-54-90-191-42.compute-1.amazonaws.com/cgi-bin/blast.cgi

Please consult the appropriate tool/API’s documentation to properly configure programmatic access.

authentication screenshot