NCBI Magic-BLAST RNA-seq mapping tool

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• Documentation
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• How Magic-BLAST works
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• Access SRA reads in the cloud
• Use NCBI SRA repository
• RNA vs DNA
• Paired reads
• Multi-threading
• Reads in FASTA or FASTQ
• Create a BLAST database
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• Release Notes

NCBI provides SRA data in the cloud to support large scale hyper parallel data analyses. Starting with version 1.6.0 Magic-BLAST downloads SRA data from the cloud providers rather than NCBI servers. Benefits include uninterrupted downloads, faster download speeds, and huge bandwidth for parallel downloads to multiple ruining processes or cloud instances. For more information see SRA in the Cloud.

To take advantage of the increased download bandwidth when running Magic-BLAST in the cloud, please configure SRA downloads, using vdb-config -i program from SRA Toolkit.

Here are webinars prepared by NCBI SRA group for how to set up a cloud instance and configure SRA downloads:

• Download SRA reads from Amazon Web Services (AWS)
• Download SRA reads from Google Cloud Platform (GCP)